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UTHSCSA Center for Surface Plasmon Resonance (CSPR)

Washing the liquid handling system

  1. WASH n washes the autosampler needle

  2. WASH s washes the injection channel in the IFC

  3. WASH l washes the sample loop (only relevant with COINJECT)

  4. WASH f washes the flow cell area in the IFC

  5. WASH i washes the injection channel, sample loop and flow cell area in the IFC. The process of washing the flow cell area may cause baseline disturbances.

  6. FLUSH and PRIME wash the pump tubing and the IFC channels at a high flow rate. This can help remove air bubbles trapped in the system.

  7. EXTRACLEAN performs an additional wash of the IFC channels immediately after an injection and may be used to minimize carryover between injections.

  8. The WASH command can be used either in the MAIN block or in an APROG in a method. FLUSH and PRIME can only be used in MAIN. EXTRACLEAN must be placed immediately after an injection and may be used in an APROG.

 
Department of Biochemistry | Graduate School of Biomedical Sciences | UTHSCSA

UTHSCSA | Graduate School of Biomedical Sciences | Department of Biochemistry | CTRC | Research

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Updated: 10/15/2009
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