Protocol: Preconditioning of Sensor Chip

In order to hydrate and clean the surface of a new sensor chip, a pre-conditioning step is performed before it is used.

You will need 1 mL of each of the following solutions pipeted into a 9 mm glass tube and capped.

• 100 mM HCl
• 50 mM NaOH (BR100358)
• 0.5% SDS (from BIAMaintenace kit stored at room temp)

Procedure:

• Place the 100 mM HCl in R2E1, the 50 mM NaOH in R2E2, and the 0.5% SDS in R2E3.
• From the menu, select Run:Run sensorgram. Set the flow rate to 100 uL/min
• Once the sensorgram starts to run, a command queue will appear.
• From the menu, select Command:Inject and select Quickinject. Select the position of the 100 mM HCl (R2E1) and select the volume to be injected (10 uL) for a 6 second contact time. Click on start and the command should appear in the command queue. Repeat this a second time so that 100 mM HCl is injected 2X.
• From the menu, select Command:Inject and select Quickinject. Select the position of the 50 mM NaOH (R2E21) and select the volume to be injected (10 uL) for a 6 second contact time. Click on start and the command should appear in the command queue. Repeat this a second time so that 50 mM NaOH is injected 2X.
• From the menu, select Command:Inject and select Quickinject. Select the position of the ).5% SDS (R2E23) and select the volume to be injected (10 uL) for a 6 second contact time. Click on start and the command should appear in the command queue. Repeat this a second time so that 0.5% SDS is injected 2X.
• After all injections have been completed, select Run:Stop sensorgram from the top menu. Pre-conditioning has been completed and your sensorgram should be similar to the one shown above.