UTHSCSA Center for Surface Plasmon Resonance (CSPR)
Maintenance
| TOOLS | SOLUTION(S) | RUN TIME | HOW OFTEN | WHAT IT DOES |
| Close | Deionized water | 11 minutes | As required | Shuts down system for up to 5 days |
| Desorb** | 3 ml 0.5% (w/v) SDS in water (BIAdesorb solution 1) in R2F3 and 3 mls 50 mM glycine-NaOH pH 9.5 (BIAdesorb solution 2) in R2F4 | 22 minutes | Weekly or as required | Remove adsorbed proteins from autosampler and the IFC |
| Flush | Running buffer | 2.5 minutes | As required | Fast cleaning of the IFC and flow cells with buffer |
| Normalize | 70% (w/w) glycerol in deionized water (BIAnormalizing solution) | 4 minutes |
Required when
|
Normalizes the resonance signal in all flow cells to compensate for small differences between individual sensor chips |
| Prime | Running buffer | 6 minutes |
Required when
|
Primes the liquid system by flushing pumps, IFC and autosampler with buffer |
| Sanitize** | 1% sodium hypochlorite (BIAdisinfectant solution) in bottle with both pump hoses (dilute 1.5 ml concentrate with 20 ml filtered distilled water) | 45 minutes | Run once a month | Cleans pumps, IFC and autosampler from micro-organisms. For best results run DESORB before. |
| Shutdown | Deionized water and 70% (v/v) ethanol | 10 minutes | Required when shutting down the instrument for more than 5 days | The procedure flushes the IFC and flow cells with water, empties the IFC of liquid and then flushes the pumps and tubing with 70% ethanol |
| Standby | Running buffer or deionized water | Up to 4 days | As required | This procedure maintains a low flow of buffer or water through the IFC and flow cells for up to 4 days |
| Superclean (under service tools) | 4 ml each 1% acetic acid, 200 mM NaHCO3, 6 M guanidium HCl, 10 mM HCl in order plus 50°C filtered milliQ water | Approximately 1-1/2 hours | Monthly or as required | This procedure allows the cleaning of the IFC under stringent conditions and should be used only when necessary. Prior to superclean, run desorb and sanitize. After superclean, run buffer injections and analyze with Scrubber . |
| System Specific Calibration Files | Biacore 1(3304) | Biacore 2(3229) |