UTHSCSA Center for Surface Plasmon Resonance (CSPR)
Types of Injections and Volumes
| Command | Comments | Injected volume | Consumption of sample |
Max flow rate | Inj Volume | Prep Time | Finish Time | Air Segments |
|---|---|---|---|---|---|---|---|---|
| INJECT | General purpose inject5 - 325 µl | Injected vol + 30 µl | 100 µl/min | 20 100 |
80 90 |
5050 | 2 | |
| KINJECT | Low dispersion inject for kinetics and for samples sensitive to dilution with running buffer. Controlled dissociation time after sample injection. | 10 - 250 µl | Injected vol + 40 µl | 100 µl/min | 20 100 |
40 45 |
2025 | 1 |
| QUICKINJECT | Low sample consumption. Less preparation time than INJECT but more sample dispersion. | 5 - 325 µl | Injected vol + 10 µl | 100 µl/min | 20 100 |
125 125 |
4045 | 3 |
| CONINJECT | Injection of second sample immediately following the first e.g. for dissociation in the presence of ligand. Variable contact times for the first sample only. | 10 - 100 µl for first sample 15 - 250 µl for second sample |
Injected vol + 40 µl for first and second sample | 100 µl/min | 20,20 100,100 |
200 250 |
8080 | 3 |
| BIGINJECT | Large volume inject. Direct input up to 325 µl, sample loop between 326 and 425 µl, direct between 426 and 750 µl. | 426 - 750 µl | Injected vol + 52 µl | 50 µl/min | 350 700 |
400 400 |
90100 | 3 |
| MANUAL INJECT | Manually controlled injection | 1 - 100 µl loading | Loaded volume + 30 µl | 100 µl/min | Prep time for loop loading 100 µl:80s |
85 | 2 | |